MPEP § 2414.03 — Amendment Adding to, Deleting from, or Replacing Sequence Information in a “Sequence Listing XML” under 37 CFR 1.835(b) (Annotated Rules)

§2414.03 Amendment Adding to, Deleting from, or Replacing Sequence Information in a “Sequence Listing XML” under 37 CFR 1.835(b)

USPTO MPEP version: BlueIron's Update: 2025-12-31

This page consolidates and annotates all enforceable requirements under MPEP § 2414.03, including statutory authority, regulatory rules, examiner guidance, and practice notes. It is provided as guidance, with links to the ground truth sources. This is information only, it is not legal advice.

Amendment Adding to, Deleting from, or Replacing Sequence Information in a “Sequence Listing XML” under 37 CFR 1.835(b)

This section addresses Amendment Adding to, Deleting from, or Replacing Sequence Information in a “Sequence Listing XML” under 37 CFR 1.835(b). Primary authority: 37 CFR 1.831(a), 37 CFR 1.835, and 37 CFR 1.831. Contains: 4 requirements, 2 permissions, and 3 other statements.

Key Rules

Topic

Sequence Listing Format

6 rules
StatutoryInformativeAlways
[mpep-2414-03-1fc560e97a559904f8758fff]
Replacement Sequence Listing Must Include No New Matter
Note:
When replacing a sequence listing XML, the new version must not introduce any new matter.

(b) Any amendment adding to, deleting from, or replacing sequence information in a “Sequence Listing XML” submitted as required by § 1.831(a) must include:

(5) A statement that the replacement “Sequence Listing XML” includes no new matter.

37 CFR 1.77 · 37 CFR 1.831(a)Sequence Listing FormatSequence Listing Requirements
StatutoryInformativeAlways
[mpep-2414-03-ea6c7005a9f3db7426eda43a]
Sequence Listing XML Must Comply with Requirements
Note:
If pre-examination staff identifies errors in a previously submitted Sequence Listing XML, or if the application fails to comply with 37 CFR 1.831-1.834, a notice indicating the deficiencies will be issued to the applicant.

Part of the pre-examination process as well as initial review by the examiner involves identifying whether an application that discloses nucleotide and/or amino acid sequences contains a compliant “Sequence Listing XML.” If pre-examination staff identifies that a previously submitted “Sequence Listing XML” contains errors and/or the application fails to comply with 37 CFR 1.831 – 1.834, a notice indicating the deficiencies will be issued to the applicant. Similarly, upon having a new application placed on the examiner’s docket, the examiner can identify errors in any “Sequence Listing XML” and/or instances of noncompliance with 37 CFR 1.831 – 1.834 that might not have been identified by pre-examination staff. The examiner can identify the outstanding requirements on a “OPAP Routing Sheet” (Document Code “SEQREQ”) and return the application to pre-examination status for the mailing of a notice indicating the deficiencies.

Jump to MPEP Source · 37 CFR 1.831Sequence Listing FormatSequence Listing RequirementsPAIR/Patent Center Status
StatutoryPermittedAlways
[mpep-2414-03-5bb2cd81a2b26a8748cbd861]
Examiner Reviews Sequence Listing XML for Compliance
Note:
The examiner checks new applications for errors in the Sequence Listing XML and compliance with 37 CFR 1.831-1.834, returning them to pre-examination status if deficiencies are found.

Part of the pre-examination process as well as initial review by the examiner involves identifying whether an application that discloses nucleotide and/or amino acid sequences contains a compliant “Sequence Listing XML.” If pre-examination staff identifies that a previously submitted “Sequence Listing XML” contains errors and/or the application fails to comply with 37 CFR 1.831 – 1.834, a notice indicating the deficiencies will be issued to the applicant. Similarly, upon having a new application placed on the examiner’s docket, the examiner can identify errors in any “Sequence Listing XML” and/or instances of noncompliance with 37 CFR 1.831 – 1.834 that might not have been identified by pre-examination staff. The examiner can identify the outstanding requirements on a “OPAP Routing Sheet” (Document Code “SEQREQ”) and return the application to pre-examination status for the mailing of a notice indicating the deficiencies.

Jump to MPEP Source · 37 CFR 1.831Sequence Listing FormatPatent Data Management SystemsSequence Listing Requirements
StatutoryRequiredAlways
[mpep-2414-03-df2f51b91e963056bd905d8f]
Requirement for Replacing Sequence Listing XML
Note:
Submit a compliant replacement XML file, amendments to the specification, and statements addressing additions, deletions, basis, and no new matter.
Applicant response to a notice or an Office action requiring a replacement “Sequence Listing XML” would involve submitting the following:
  • (1) A compliant replacement “Sequence Listing XML” submitted as an XML file containing the entire “Sequence Listing XML,” including any additions, deletions, or replacements of sequence information, using either the USPTO patent electronic filing system (currently, XML files can only be submitted via Patent Center) or a read-only optical disc that is mailed or hand-delivered into the Office. If the size of “Sequence Listing XML” file exceeds the upload limit of Patent Center, then it must be submitted on read-only optical disc (37 CFR 1.835(b)(1));
  • (2) An amendment to the specification to incorporate by reference the material in the replacement “Sequence Listing XML” by reciting in a separate paragraph of the specification the name of the file, the date of creation, and the size of the file in bytes (37 CFR 1.835(b)(1) and 37 CFR 1.835(c));
  • (3) A statement that identifies the location of all additions, deletions, or replacements of sequence information relative to the replaced “Sequence Listing XML” (37 CFR 1.835(b)(3));
  • (4) A statement identifying the basis for the additions, deletions, or replacements of the sequence information in the application as originally filed for all amended sequence data in the replacement “Sequence Listing XML,” which would include, e.g., paragraphs of specification or sheets of the drawings where a sequence is disclosed, identifying the sequence which is part of the replacement “Sequence Listing XML” (37 CFR 1.835(b)(4)); and
  • (5) A statement that no new matter is introduced by the content of the replacement “Sequence Listing XML” (37 CFR 1.835(b)(5)).
Jump to MPEP Source · 37 CFR 1.835Sequence Listing FormatExamination ProceduresSequence Listing Requirements
StatutoryRequiredAlways
[mpep-2414-03-b1e75a1eef86ea57701321aa]
No New Matter in Sequence Listing XML Replacement
Note:
The rule requires that when replacing a 'Sequence Listing XML' in patent applications, no new matter should be introduced.

Applicant response to a notice or an Office action requiring a replacement “Sequence Listing XML” would involve submitting the following:

(5) A statement that no new matter is introduced by the content of the replacement “Sequence Listing XML” (37 CFR 1.835(b)(5)).

Jump to MPEP Source · 37 CFR 1.835Sequence Listing FormatSequence Listing RequirementsAmendments Adding New Matter
StatutoryRequiredAlways
[mpep-2414-03-b101602f01c3912b14bdefbe]
Replacement Sequence Listing XML Required
Note:
If a deficiency is recognized in a previously submitted Sequence Listing XML, an amended version must be filed including all required items.

If applicant recognizes a deficiency in a previously submitted “Sequence Listing XML” or otherwise chooses to amend a previously submitted “Sequence Listing XML,” a replacement “Sequence Listing XML” must be filed including all of the above-described items.

Jump to MPEP Source · 37 CFR 1.835Sequence Listing FormatSequence Listing Requirements
Topic

Sequence Listing Content

2 rules
StatutoryInformativeAlways
[mpep-2414-03-930872e8bc76afa453dc1da6]
Requirement for Sequence Disclosure After July 1, 2022
Note:
This rule requires all applications with a filing date on or after July 1, 2022, that disclose nucleotide and/or amino acid sequences to include such disclosure in the sequence listing.

[Editor Note: This section is applicable to all applications with a filing date, or, for national phase applications, an international filing date, on or after July 1, 2022, having disclosure of one or more nucleotide and/or amino acid sequences as defined in 37 CFR 1.831(b).]

37 CFR 1.77 · 37 CFR 1.831(b)Sequence Listing ContentSequence Listing RequirementsSequence Listing Format
StatutoryInformativeAlways
[mpep-2414-03-64a0059822c45ee01dce4746]
Sequence Listing XML Required for Nucleotide and Amino Acid Sequences
Note:
The application must include a compliant Sequence Listing XML if it discloses nucleotide or amino acid sequences, to be reviewed by pre-examination staff and examiners.

Part of the pre-examination process as well as initial review by the examiner involves identifying whether an application that discloses nucleotide and/or amino acid sequences contains a compliant “Sequence Listing XML.” If pre-examination staff identifies that a previously submitted “Sequence Listing XML” contains errors and/or the application fails to comply with 37 CFR 1.831 – 1.834, a notice indicating the deficiencies will be issued to the applicant. Similarly, upon having a new application placed on the examiner’s docket, the examiner can identify errors in any “Sequence Listing XML” and/or instances of noncompliance with 37 CFR 1.831 – 1.834 that might not have been identified by pre-examination staff. The examiner can identify the outstanding requirements on a “OPAP Routing Sheet” (Document Code “SEQREQ”) and return the application to pre-examination status for the mailing of a notice indicating the deficiencies.

Jump to MPEP Source · 37 CFR 1.831Sequence Listing ContentSequence Listing FormatSequence Listing Requirements
Topic

Statement Under Article 19

1 rules
StatutoryRequiredAlways
[mpep-2414-03-d31eebb8e234e086c9a57abd]
Amendment to Sequence Listing XML Must Include Updated File and References
Note:
When amending a Sequence Listing XML, the updated file must be submitted along with a request to amend the specification and references to the original application.
(b) Any amendment adding to, deleting from, or replacing sequence information in a “Sequence Listing XML” submitted as required by § 1.831(a) must include:
  • (1) A replacement “Sequence Listing XML” in accordance with the requirements of §§ 1.831 through 1.834 containing the entire “Sequence Listing XML,” including any additions, deletions, or replacements of sequence information, which shall be submitted:
    • (i) Via the USPTO patent electronic filing system; or
    • (ii) On a read-only optical disc, in compliance with § 1.52(e), labeled as “REPLACEMENT MM/DD/YYYY” (with the month, day, and year of creation indicated);
  • (2) A request to amend the specification to include an incorporation by reference statement of the material in the replacement “Sequence Listing XML” file that identifies the name of the file, the date of creation, and the size of the file in bytes (see § 1.77(b)(5)(ii)), except when the replacement “Sequence Listing XML” is submitted to the United States International Preliminary Examining Authority for an international application;
  • (3) A statement that identifies the location of all additions, deletions, or replacements of sequence information relative to the replaced “Sequence Listing XML”;
  • (4) A statement that indicates the support for the additions, deletions, or replacements of the sequence information, with specific references to particular parts of the application as originally filed (specification, claims, drawings) for all amended sequence data in the replacement “Sequence Listing XML”; and
  • (5) A statement that the replacement “Sequence Listing XML” includes no new matter.
37 CFR 1.77 · 37 CFR 1.831(a)Statement Under Article 19PCT Article 19 AmendmentsSequence Listing Format
Topic

PAIR/Patent Center Status

1 rules
StatutoryPermittedAlways
[mpep-2414-03-1f54d730bed634db03023fa3]
Examiner Can Identify Sequence Listing Errors
Note:
The examiner can identify errors in a sequence listing and return the application to pre-examination status for a notice of deficiencies.

Part of the pre-examination process as well as initial review by the examiner involves identifying whether an application that discloses nucleotide and/or amino acid sequences contains a compliant “Sequence Listing XML.” If pre-examination staff identifies that a previously submitted “Sequence Listing XML” contains errors and/or the application fails to comply with 37 CFR 1.831 – 1.834, a notice indicating the deficiencies will be issued to the applicant. Similarly, upon having a new application placed on the examiner’s docket, the examiner can identify errors in any “Sequence Listing XML” and/or instances of noncompliance with 37 CFR 1.831 – 1.834 that might not have been identified by pre-examination staff. The examiner can identify the outstanding requirements on a “OPAP Routing Sheet” (Document Code “SEQREQ”) and return the application to pre-examination status for the mailing of a notice indicating the deficiencies.

Jump to MPEP Source · 37 CFR 1.831PAIR/Patent Center StatusPatent Data RetrievalSequence Listing Content

Citations

Primary topicCitation
Statement Under Article 1937 CFR § 1.52(e)
Statement Under Article 1937 CFR § 1.77(b)(5)(ii)
PAIR/Patent Center Status
Sequence Listing Content
Sequence Listing Format
Statement Under Article 19
37 CFR § 1.831
Sequence Listing Format
Statement Under Article 19
37 CFR § 1.831(a)
Sequence Listing Content37 CFR § 1.831(b)
Sequence Listing Format37 CFR § 1.835(b)(1)
Sequence Listing Format37 CFR § 1.835(b)(3)
Sequence Listing Format37 CFR § 1.835(b)(4)
Sequence Listing Format37 CFR § 1.835(b)(5)
Sequence Listing Format37 CFR § 1.835(c)

Source Text from USPTO’s MPEP

This is an exact copy of the MPEP from the USPTO. It is here for your reference to see the section in context.

BlueIron Last Updated: 2025-12-31