How should a “Sequence Listing” be submitted electronically to the USPTO?

According to MPEP 2425, a “Sequence Listing” can be submitted electronically to the USPTO as follows:

• The ASCII plain text file must be submitted via the USPTO patent electronic filing system
• The file must not exceed 100 MB
• File compression is not permitted for electronic submissions

As stated in 37 CFR 1.824(b)(1), “Electronically via the USPTO patent electronic filing system, where the file must not exceed 100 MB, and file compression is not permitted.” This means that applicants must ensure their Sequence Listing file is within the size limit and is not compressed when submitting electronically.

Rule Not Applicable to Post-July 1, 2022 Filings MPEP 2425 Informative
Sequence Listing File Must Conform to ASCII Plain Text Requirements MPEP 2425 Required
Sequence Listing Must Be Continuous ASCII Text MPEP 2425 Required
Single Sequence Listing Required MPEP 2425 Required
Sequence Listing Can Be Split Across Discs MPEP 2425 Permitted