How should a “Sequence Listing” be submitted electronically to the USPTO?

This page is an FAQ based on guidance from the Manual of Patent Examining Procedure. It is provided as guidance, with links to the ground truth sources. This is information only: it is not legal advice.

According to MPEP 2425, a “Sequence Listing” can be submitted electronically to the USPTO as follows:

• The ASCII plain text file must be submitted via the USPTO patent electronic filing system
• The file must not exceed 100 MB
• File compression is not permitted for electronic submissions

As stated in 37 CFR 1.824(b)(1), “Electronically via the USPTO patent electronic filing system, where the file must not exceed 100 MB, and file compression is not permitted.” This means that applicants must ensure their Sequence Listing file is within the size limit and is not compressed when submitting electronically.